In the present study, an attempt was taken for rapid evaluation of iron-reduction capacity and growth of Shewanella algae through optimization of process parameters using Taguchi methodology. Therefore, proper evaluation of screening protocol is imperative benchmarks for selection of biocatalyst in MFC. The microorganism is one of the key limiting factors to enhance the performance efficiency of MFC. Microbial fuel cells (MFCs) are considered one of the most promising alternative energy generation systems. When the organism was cultured under the optimal condition using casein as a nitrogen source and glucose as the carbon source, at 40 0C and pH 9, 1590 ng/mL of enzyme production was estimated.ĭue to energy expansion and sustainable development of resources and systems, there has been an increasing demand for environmentally friendly alternative energy systems. Regarding the optimal conditions, the suitable temperature for maximum enzyme production was found to be 40 0C and pH was 9. The optimal carbon and nitrogen sources were identified to be Glucose and casein respectively. The organism which was isolated from soil and capable of producing the caseinase enzyme was identified to be Bacillus subtilis based on the Biochemical tests and 16S rRNA sequencing result. The study also includes its kinetic characterization using the parameters Temperature, pH as well as Carbon and Nitrogen Sources. This is followed by the production of enzyme and its purification. In view of the importance of caseinase, the current research deals with the isolation and identification of caseinase producing bacteria from soil. Caseinase enzyme can be extensively used at the industrial scale for Milk, Textile, Dairy, Paper industry and several other medical purposes. Casein can be an instant energy source to the body and involves in muscle building.
Caseinase is involved in the breakdown of milk protein casein and converts casein into smaller simple sugars which can be easily utilized by the body for the production of ATP and Fat.
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